2 edition of ultrastructural and molecular characterization of ciliogenesis in Tetrahymena thermophila found in the catalog.
ultrastructural and molecular characterization of ciliogenesis in Tetrahymena thermophila
Darrell Lee Gitz
Written in English
|Statement||by Darrell Lee Gitz|
|The Physical Object|
|Pagination||viii, 137 leaves :|
|Number of Pages||137|
The single Tetrahymena D2IC gene (accession no. ABX) encodes a protein of amino acids with a molecular weight of 68, Da. Tetrahymena D2IC includes four WD40 repeats, which are characteristic of dynein by: Abstract. Tetrahymena cells (Fig. 1) have upwards of cilia depending upon the species and the stage in the cell cycle (Nanney, ). Of these, about 20% are in the oral apparatus (Williams and Bakowska, ). The cilia are easily detached (less so the oral cilia) by the application of mild shearing forces after various chemical treatments (reviewed by Nozawa, ).Cited by: 5.
Genetic characterization of the secretory mutant MS-1 of Tetrahymena thermophila: vacuolarization and block in secretion of lysosomal hydrolases are caused by a Cited by: Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics , – Medline, Google Scholar; Chau, M.F., and Orias, E. (). Developmentally programmed DNA rearrangement in Tetrahymena thermophila: isolation and sequence characterization of three new alternative deletion systems. Biol.
Recent Updates. Recent Papers Nabeel-Shah S, Ashraf K, Saettone A, Garg J, Derynck J, Lambert JP, Pearlman RE, Fillingham J () Nucleus-specific linker histones Hho1 and Mlh1 form distinct protein interactions during growth, starvation and development in Tetrahymena thermophila. In contrast to cycling cells, multiciliated cells have the ability to assemble hundreds of centrioles. EM shows that these centrioles arise through two parallel pathways initiated in the vicinity of the cell's existing centrosome (Dirksen, ; Hagiwara et al., ).In the centriolar pathway, multiple new centrioles form around an existing mother centriole, similar to the process in cycling Cited by:
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Tetrahymena thermophila is a free-swimming ciliate that utilizes hundreds of motile cilia for hydrodynamic force-generation. Tetrahymena belong to the superphylum Alveolata which also contains the parasitic Apicomplexans and the aquatic Dinoflagellates and together compose one of the largest groups of the kingdom Protozoa [ 1 ].Cited by: Tetrahymena thermophila is an excellent model organism for the study of cilia and ciliogenesis.
The cell is covered by about 1, cilia which are essential for survival. Additionally, the Tetrahymena genome is available and targeted genetic manipulations are straightforward. In this chapter, we describe five protocols that examine properties of cilia: (a) measuring mRNA levels to Cited by: 7.
Tetrahymena is a large and complex cell yet a rather small and simple ciliate. Its normal dimensions are about 50 μm in length and 20 μm in maximum ymena thermophila (T. thermophila) possesses a diploid micronucleus (Mi) possessing two complete haploid genome sets and capable of both mitosis and meiosis, plus a macronucleus (Ma) that is made up of multiple copies of a rearranged Cited by: Tetrahymena thermophila is a ciliate with hundreds of cilia primarily used for cellular motility.
These cells propel themselves by generating hydrodynamic forces through coordinated ciliary beating. A key technological advance in recent years with Tetrahymena thermophila studies is the application of green fluorescent protein (GFP) and its spectral variants, which have been used to create fusion proteins labeled with different colors in the same cell ().With this advance comes the ability to visualize the assembly and structural localization of proteins and by: KIF17 is the mammalian homolog of the molecular motor OSM-3 (osmotic avoidance abnormal protein 3; a dendritic motor for odorant receptors) in C.
elegans and Kin5 in Tetrahymena thermophila. CHAPTER 16 Regulated Protein Secretion in Tetrahymena thermophila Aaron P. Turkewitz, N. Doane Chilcoat, Alex Haddad, and John W. Verbsky Department of Molecular Genetics and Cell Biology The University of Chicago Chicago, Illinois I.
Introduction by: We describe the protocol through which we identify and characterize dynein subunit genes in the ciliated protozoan Tetrahymena thermophila. The gene(s) of interest is found by searching the Tetrahymena genome, and it is characterized in silico including the prediction of the open reading frame and identification of likely introns.
The gene is then characterized experimentally, including the confirmation Cited by: 3. Ciliogenesis: building the cell's antenna proteins is another key regulator of ciliogenesisthe molecular basis of which is for assembly of the axoneme in Tetrahymena by: PESTICIDE BIOCHEMISTRY AND PHYSIOL () Characterization of a Xenobiotic Thiol Methyltransferase and Its Role in Detoxication in Tetrahymena thermophila ANNAMARIE DROTAR AND RAY FALL' Cooperative Institute for Research in Environmental Sciences, and Department of Chemistry and Biochemistry, Campus BoxUniversity of Colorado, Boulder, Colorado Received J ; accepted Septem The protozoan Tetrahymena thermophila Cited by: Abstract.
Tetrahymena thermophila is a ciliate with hundreds of cilia primarily used for cellular motility. These cells propel themselves by generating hydrodynamic forces through coordinated ciliary beating. The coordination of cilia is ensured by the polarized organization of basal bodies (BBs), which exhibit remarkable structural and molecular conservation with BBs in other by: Centrin, an EF hand Ca(2+) binding protein, has been cloned in Tetrahymena thermophila.
It is a amino acid protein of kDa with a unique N-terminal region, coded by a single gene. Frankel J. Cell biology of Tetrahymena thermophila.
Methods in Cell Biology: Tetrahymena thermophila Asai J.D. Academic PressSan Diego (in press). Google Scholar; 24 Gaertig J., Gorovsky M. Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc. Natl. Acad.
Sci. Purchase Cilia and Flagella, Volume 47 - 1st Edition. Print Book & E-Book. ISBNCiliogenesis is defined as the building of the cell's antenna or extracellular fluid mediation mechanism.
It includes the assembly and disassembly of the cilia during the cell cycle. Cilia are important organelles of cells and are involved in numerous activities such as cell signaling, processing developmental signals, and directing the flow of fluids such as mucus over and around cells.
Due to the importance of these cell processes, defects in ciliogenesis TH: H We cloned two genes, KIN1 and KIN2,encoding kinesin-II homologues from the ciliate Tetrahymena thermophila and constructed strains lacking eitherKIN1 or KIN2 or both genes. Cells with a single disruption of either gene showed partly overlapping sets of defects in cell growth, motility, ciliary assembly, and thermoresistance.
Tetrahymena thermophila is a ciliate with a highly organized, microtubule based cytoskeleton. Each cell contains, basal bodies, either in cortical rows or in the feeding structure known as the. Tetrahymena thermophila is a free-swimming ciliate that utilizes hundreds of motile cilia for hydrodynamic force-generation.
Tetrahymena belong to the superphylum Alveolata which also contains the parasitic Apicomplexans and the aquatic Dinoflagellates and together compose one of the largest groups of the kingdom by: Purchase Methods in Cilia and Flagella, Volume - 1st Edition.
Print Book & E-Book. ISBNMetal(loid)s' accumulation and interactions were studied in Tetrahymena thermophila after 24 h exposure to µM CuI, 20 µM CdII, 1 µM of AgI, HgII, PbII, AsIII, AsV, SbIII, SbV, SeIV, or SeVI. We report that the conserved WD40 repeat domain–containing cartwheel protein Poc1 is required for the structural maintenance of centrioles in Tetrahymena thermophila.
Furthermore, human Poc1B is required for primary ciliogenesis, and in zebrafish, DrPoc1B knockdown causes ciliary defects and morphological phenotypes consistent with human Cited by: Molecular Biology of the Cell Vol. 10, No. 10 Articles Free Access Kinesin-II Is Preferentially Targeted to Assembling Cilia and Is Required for Ciliogenesis and Normal Cytokinesis in Tetrahymena .The mature culture contains many fully ciliated cells (Fig.
1 B) with occasional cells at earlier steps of day ALI + 14 cultures, typically 40–60% of cells are ciliated, consistent with a previous report (Toskala et al., ).Transmission EM (TEM) of day ALI + 2 cultures revealed deuterosomes and fibrous granules in the apical cytoplasm of ciliating cells (Fig.
1 C), similar Cited by: